Please use this identifier to cite or link to this item:
Title: Transcriptome Assembly of the Bast Fiber Crop, Ramie, Boehmeria nivea (L.) Gaud. (Urticaceae) Louay
Authors: Louay Al-Ani 1, 2 and Michael K. Deyholos 3
Keywords: bast; fiber; pectin; ramie; transcriptome; Urticaceae
Issue Date: 1-Feb-2018
Abstract: Ramie (Boehmeria nivea) is a perennial crop valued for its strong bast fibers. Unlike other major bast fiber crops, ramie fiber processing does not include retting, but does require degumming, suggesting distinctive features in pectin and the development and composition of fibers. A comprehensive transcriptome assembly of ramie has not been made available, to date. We obtained the sequence of RNA transcripts (RNA Seq) from the apical region of developing ramie stems and combined these with reads from public databases for a total of 157,621,051 paired-end reads (30.3 billion base pairs Gbp) used as input for de novo assembly, resulting in 70,721 scaffolds ( 200 base pairs (bp); N50 = 1798 bp). As evidence of the quality of the assembly, 36,535 scaffolds aligned to at least one Arabidopsis protein (BLASTP e-value 10􀀀10). The resource described here for B. nivea will facilitate an improved understanding of bast fibers, cell wall, and middle lamella development in this and other comparative species.
Appears in Collections:البحوث العلمية

Files in This Item:
File Description SizeFormat 
Transcriptome_Assembly_of_the_Bast_Fiber_Crop_Rami.pdf4.27 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.