Please use this identifier to cite or link to this item: http://37.48.73.109:8080/jspui/jspui/handle/123456789/1369
Title: Leishmania donovani, Ascophyllum nodosum, liver, MTT
Authors: Abdulkhaliq Roua Jamal1, Mohammed Sabaa Taher2, Abbas Ahmed Abdul-Hassan3 and Muhaidi M.J.4*
Keywords: HLA-G, frequent abortion, polymorphism, husband's relatives, husband's strange.
Issue Date: 2019
Publisher: Research Journal of Biotechnology
Abstract: The 14 bp insertion/deletion gene polymorphism of insertion /deletion (+14 bp -14 bp) in the exon 8 of (HLA-G) human leucocyte antigen-G gene has been usually recognized to associate with frequent abortion (RA). This study was designed to determine the possible association between 14bp of HLA-G deletion/ insertion gene polymorphism with frequent abortion in husband's relatives and husbands strange. Peripheral blood was collected from ninety (90) women with periodic abortion, three abortions or more with unknown causes (husband's relatives was 58 and husband's strange was 32). DNA from the peripheral blood was extracted, the concentration and purity of DNA were measured then, DNA primer was designed by using genious software program. HLA-G gene of the both groups was amplified by using polymerase chain reaction (PCR) and analyzed by gel electrophoresis. The result revealed that homozygous insertion (+14bp/+14bp) was in husband's relatives (13), and husband's strange (11), and homozygous deletion (- 14bp/-14bp) results were in husband's relatives (11), and husband's strange (5) while heterozygous (+14bp/- 14bp) results were in husband's relatives (34), and husband's strange (16). In the present study any significant variation was not found between heterozygous and homozygous amongst husband's relatives and husbands strange in Iraqi women. We also did not found any implication between husband's relatives and husbands strange in the field of alleles (+14bp (insertion) or -14bp (deletion) in recurrent abortion).
URI: http://37.48.73.109:8080/jspui/jspui/handle/123456789/1369
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