Isolation and Characterization of Phenazine Produced by SP9 Strain of Pseudomonas aeruginosa

Abstract

A Pseudomonas strain, designated SP9, was isolated from clinical specimens and identified as Pseudomonas aeruginosa based on morphology; conventional biochemical and physiologic tests. One antimicrobial substances produced by this strain proved to be phenazine bio-product, based on TLC, HPLC and IR. For purpose of studying of the antimicrobial activity, gel filtration was employed in the purification of phenazine. The bio-product is effective against Bacillus subtillus, Staphylococcus aureus, Staphylococcus epidermidis , Escherichia coli and Salmonella typhi and Candida albicans.